Development of compact table grape plants via CRISPR-directed dominant mutation in VvDELLA1
Research project 6
Project researcher: Dr. Etti Or
Developing a compact orchard is an attractive target that may reduce the complication and cost of various practices, change the balance between vegetative and reproductive mass and improve land, water, and fertilizers usage. The translation of this motivation to practice was restricted to the use of dwarfing rootstocks and Gibberellin (GA) biosynthesis inhibitors mainly, with limited effects and undesired side effects. The CRISPR/Cas9 technology can revolutionize this area by modifying GA signaling components. The challenging aspect is identifying a manipulation strategy that will limit vegetative growth with minimal effects on fruit quality and productivity. Based on very promising findings, we hope that such an approach can be implemented into table grapes via CRISPR/Cas9 guided manipulation of the DELLA genes.
Research project Etti Or
Establishment of the transient in vivo system in grapevine using GFP marker gene
Research project Etti Or
Examination of regeneration ability of the current grape pre-embryonic cultures
Research project Etti Or
New pre-embryonic cultures establishment from grapevine anthers
Beyond the advantages listed above, such manipulation applied to table grape can turn it into “grapmato” with year-round production and potential transformation into an intensive annual or biannual crop. Such an approach may yield additional commercial benefits beyond the abilities of our limited imagination. Therefore, the project’s objective is to generate dominant mutation in the VvDELLA1 gene via CRISPR-directed gene editing that will lead to the production of the compact grapevine with alternative growth and yield pattern.
אתי אור

Dr. Etti Or
Project researcher

Plant genome editing, Agricultural Research Organization, Volcani Institute.

Email: vhettior@volcani.agri.gov.il

Phone: +972-3-9683345

Website: Dr. Etti Or

Research areas:

  • Grapevine molecular physiology. Grapevine bud development, mechanism of grapevine bud dormancy release and regulation of grapevine fruitfulness.
  • Regulation of berry size.

Research partners:

Dr. Elena Marchevka

Dr. Elena Marchevka
Ph.D. – Plant genome editing specialist

Email: elenam@volcani.agri.gov.il

Research areas:
Plant genome editing.

institution:
Plant genome editing, Agricultural Research Organization, Volcani Institute.

לילך פרקש-חיים

Lilach Farkash-Haim
M.Sc. – Plant tissue culture specialist

Research areas:
plant cultures.

institution:
Plant genome editing, Agricultural Research Organization, Volcani Institute.

Tatyana Okun

Tatyana Okon
Plant tissue culture technician

Research areas:
plant cultures.

institution:
Plant genome editing, Agricultural Research Organization, Volcani Institute.