Genome editing technologies: transformation of recalcitrant plants
Research project 18
Project researcher: Prof. Alexander Vainstein

Traditional approaches of DNA delivery involve a stage of regeneration in tissue culture to which many plants/varieties appear to be recalcitrant, and regeneration events are extremely rare if at all possible.

Ectopic expression of factors involved in hormonal balance and embryogenesis/organogenesis will be tested. These have been previously demonstrated to aid the induction of regeneration in several plant species. Alternatively, DNA delivery that includes various nanoparticles as nano-carriers of biological molecules to transform pollen grains will be explored.

Transgenic pollen grains could then be used to pollinate flowers and create transgenic seeds without the need for regeneration and lengthy in-vitro techniques.

מחקר מס' 18 - אלכסנדר ויינשטיין
Tobacco and pepper pollen 18 hrs after bombardment with particles coated with a plasmid carrying LAT52:GFP.
Prof. Alexander Vainstein

Prof. Alexander Vainstein
Project researcher

Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem.

Email: alexander.vainstein@mail.huji.ac.il

Phone: +972-8-9489082

Website: Prof. Alexander Vainstein

Research areas:

  • Site-specific genome modification and genetic engineering in plants.
  • Genomic/metabolomic/proteomic approaches for identification of novel aroma genes.
  • Metabolic engineering of plants and yeast.

Research partners:

ד"ר ירין אקנין

Yarin Aknin
PhD student

Institution:
Plant Sciences and Genetics in Agriculture, The Robert H Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem.

Nir Kaofer
MSc student

Institution:
Plant Sciences and Genetics in Agriculture, The Robert H Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem.