New tools for transgene excision, using Cas9-FOK1 cleavage to target the T-DNA termini
Research project 20
Project researcher: Dr. Ben Rimon

Genome editing using Cas9 is becoming an essential practice in accelerated breeding programs.

T-DNA (that holds all the machinery for genome editing) exclusion is a mandatory step towards regulating final cultivars as non-GMO. Mendelian segregation is used to segregate out the T-DNA in cultivars propagated by seeds. In vegetatively propagated plants such as fruit trees or ornamentals, excision of the T-DNA that induced the mutagenesis is more challenging.

Exclusion of the T-DNA by dCas9-FOKI that will induce double strand break downstream to the sgRNA recognition site

Our research aims to develop a molecular technology for T-DNA excision without crossing it out. This technology is based on recombinant Cas9 that can induce site-specific DNA breaks outside the DNA recognition site. The development of this technology will pave the way for integrating genome editing procedures in accelerated breeding programs. It will allow keeping the elite characteristics of background cultivars used without the final product specified as GMO.

Project researcher:

Dr. Ben Rimon

Dr. Ben Rimon
Project researcher

Plant Sciences, Ornamental Plants and Agricultural Biotechnology, Agricultural Research Organization, Volcani Institute.

Email: benrimon@agri.gov.il

Phone: +972-3-9683066

Website: Dr. Ben Rimon

Laboratory website

Research areas:

  • Studying of flowering mechanisms and plant sexual reproduction.
  • Development of genomic editing technologies to improve plant selected traits.

Research partner:

ד"ר ארצ'יט סוד

Dr. Archit Sood
Postdoctoral researcher

Research areas:
The effect of environmental conditions on flower development and control of Chrysanthemum sexual reproduction.

Institution:
Plant Sciences, Ornamental Plants and Agricultural Biotechnology, Agricultural Research Organization, Volcani Institute.